Characterization of excretory-secretory antigens of adult Toxocara canis by western blotting
نویسندگان
چکیده
منابع مشابه
Evaluate Toxocara Canis Excretory-Secretory Antigens in Experimental Allergic Encephalomyelitis (EAE)
BACKGROUND: Toxocara canis is the most prevalent intestinal roundworm of canid species. OBJECTIVES: This study aims to evaluate the effects of Toxocara canis excretory-secretory antigens (TcES Ag) on modulating the immune system in Experimental Allergic Encephalomyelitis (EAE) model. METHODS: Adult worms of T.canis were collected from dogs to obtain excretory-secretory antigens. Female C57BL/6m...
متن کاملSandwich ELISA detection of excretory-secretory antigens of Toxocara canis larvae using a specific monoclonal antibody.
We produced a new monoclonal antibody (mAb) to the excretory-secretory (ES) antigens of Toxocara canis larvae. The mAb (IgG1) reacts specifically with the 120 kDa protein of many ES molecules and does not have any cross-reactivity with adult T. canis antigens. Sandwich ELISA to detect the ES antigens was performed using the mAb and rabbit polyclonal antiserum. The lower limit for the detection ...
متن کاملAn alternate technique for isolation of Toxocara canis excretory- secretory antigens
The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluate...
متن کاملEvaluate Toxocara canis excretory-secretory antigens in experimental allergic encephalomyelitis (EAE)
BACKGROUND: Toxocara canis is the most prevalent intestinal roundworm of canid species. OBJECTIVES: This study aims to evaluate the effects of Toxocara canis excretory-secretory antigens (TcES Ag) on modulating the immune system in Experimental Allergic Encephalomyelitis (EAE) model. METHODS: Adult worms of T.canis were collected from dogs to obtain excretory-secretory antigens. Female C57BL/6 ...
متن کاملAntigenic Characterization of Excretory / Secretory Antigen of Oesophagostomum Columbianum Using Western Blotting
In the present investigation, E/S antigen from O. columbianum was isolated by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, characterization, the E/S antigen revealed six polypeptides at 29.0, 38.0, 49.0, 71.0, 84.0 and 95.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 38.0 and 49.0 kDa were detected in western bl...
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ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2012
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-012-0209-x